Application

Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:whole-mount in situ hybridizationfluorescence in situ hybridizationenzyme linked immunosorbent assay (ELISA)dot blotSouthern blotwestern blotdouble labeling experimentslectin blotsAnti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.

General description

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Dot blot: 50 to 100 mU/ml ELISA: 2 to 50 mU/ml Western blot: 50 to 100 mU/ml Southern blot: 50 to 100 mU/mlWorking solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Reconstitution

Add 1 ml double-distilled water to a final concentration of 50 U/ml.

Specificity

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.